RNA Editing- 2024 Project
The Project
A: Diagram of CRISPR_based deletion of 12 nucleotides in gene of Sindbis-GFP virus. B: Quantification of cleavage and ligation efficiency with viral RNA.
Despite the extensive progress that has been made in DNA editing, tools to manipulate RNA, without a DNA intermediate, are limited. Manipulating RNA is a prerequisite for studying RNA viruses, and to support the growing interest in vaccine development and gene therapy.
Combining sequence-specific RNA cleavage by CRISPR RNA-guided ribonucleases with programmable RNA repair to make precise deletions, insertions, and substitutions, Blake's team aims to modify any RNA target. Having the ability to edit RNA will unleash a myriad of applications, starting with its use by researchers in the fields of drug discovery and vaccine development.
The Impact
- This novel method has applications for gene therapy companies, vaccine development, and academic research
- This team has demonstrated this method can be used to remove non-sense mutation common in cystic fibrosis patients
- One PCT provisional patent has been filed on this technology!
Meet the Team
Dr. Blake Wiedenheft
Project Lead & Professor
Microbiology and Immunology, Biotechnology
